Task 2, Applying Talairach VOIs

The Talairach atlas includes five levels:

Talairach Level 1 -Main structures (left, right cerebrum, cerebellum, brain stem, and so on)

Talairach Level 2-Lobes (temporal, frontal, parietal, posterior, occipital, limbic, anterior, midbrain, and so on)

Talairach Level 3-Gyri (temporal, precentral, fusiform, thalamus, ventricles, and so on)

Talairach Level 4-Matter (white matter, gray matter, CSF)

Talairach Level 5-Brodmann areas (areas 1-47, hippocampus, putamen, and so on)

To apply Talairach VOIs

1 Select the Talairach-aligned image.

2 Click VOI > Open VOI in the MIPAV window. The Open VOI dialog box appears.

3 Navigate to the directory where you saved the Talairach atlas (Figure 1).

4 Click Talairach atlas folder (Figure 1A). The Talairach level folders open (Figure 1B).

5 Click one of the Talairach level folders that you want to use (Figure1B). The folder opens showing all of the applicable VOIs for that level (Figure 1C).

6 Select one of the VOIs.

7 Click Open. The selected VOI appears on the Talairach-aligned image (Figure 2a).

8 Repeat steps 2-step 7 until all of the VOIs that you want to see are displayed on the image (Figure 2).

Note: The Talairach transformation deforms the brain. Measurements are not correct in Talairach space.

Figure 1. Navigating to the correct Talairach folder: (A) the Talairach folder; (B) the five levels of Talairach folders, and (C) the VOIs in the Talairach level 1 folder

Figure 2. The sequence of images that results as you apply each desired VOI one at a time You can add as few as one or as many VOIs as you want to appear on the image. Note that each VOI appears in a unique color.

Task 3, Segmenting the original image