Class AlgorithmAutoCovariance

  • All Implemented Interfaces:
    java.awt.event.ActionListener, java.awt.event.WindowListener, java.lang.Runnable, java.util.EventListener

    public class AlgorithmAutoCovariance
    extends AlgorithmBase
    let deli(x,y) = (i(x,y) - )/ where the angle brackets are used to denote a spatial average. Then the two-dimensional autocovariance function, g(e,n) is defined as g(e,n) = which for a M by P discrete data set is calculated as g(e,n) = (num/denom) - 1 where num = (1/(M - e)(P - n))sum from j = 1 to M-e sum from k = 1 to P-n for: i(j,k)*i(j+e,k+n) denom = [(1/(2(M-e)(P-n))) sum from j = 1 to M sum from k = 1 to P of i(j,k) + i(j+e,k+n)]**2 Note that while some medical literature refers to this as a autocorrelation, it is actually an autocovariance since means are subtracted. 3 articles in which this formula are used: 1.) "Two-photon image correlation spectroscopy and image cross-correlation spectroscopy" by P. W. Wiseman, J.A. Squier, M.H. Ellisman, and K.R. Wilson, Journal of Microscopy, Vol. 200. Pt. 1, October 2000, pp. 14-25. 2.) "Quantitation of Membrane Receptor Distributions by Image Correlation Spectroscopy: Concept and Application" by Nils O. Petersen, Pia L. Hoddelius, Paul W. Wiseman, Olle Seger, and Karl-Eric Magnusson, Biophysical Journal, Volume 65, September, 1993, pp. 1135-1146. 3.) "Image cross-correlation spectroscopy: A new experimental bipohysical approach to measurement of slow diffusion of fluorescent molecules" by Mamta Srivastava & Nils O. Petersen, Methods in Cell Science, Vol 18, March, 1996, pp. 47-54.

    The autocovariance fits to a function of the form a0 + a1*exp(-(x**2 + y**2)/w**2) = a0 + a1*exp(a2*distSqr), where a2

    • Field Detail

      • destImageB

        private ModelImage destImageB
        DOCUMENT ME!
      • destImageG

        private ModelImage destImageG
        DOCUMENT ME!
      • destImageR

        private ModelImage destImageR
        DOCUMENT ME!
      • fwhm

        private int fwhm
        full width at half maximum of the autocovariance.
      • fwhmB

        private int fwhmB
        DOCUMENT ME!
      • fwhmG

        private int fwhmG
        DOCUMENT ME!
      • fwhmR

        private int fwhmR
        DOCUMENT ME!
    • Constructor Detail

      • AlgorithmAutoCovariance

        public AlgorithmAutoCovariance​(ModelImage destImg,
                                       ModelImage srcImg)
        Constructor for black and white image in which covariance coefficients are placed in a predetermined destination image.
        Parameters:
        destImg - Image model where result image is stored.
        srcImg - Source image model.
      • AlgorithmAutoCovariance

        public AlgorithmAutoCovariance​(ModelImage destImageR,
                                       ModelImage destImageG,
                                       ModelImage destImageB,
                                       ModelImage srcImg)
        Constructor for color image in which covariance coefficients are placed in predetermined destination images.
        Parameters:
        destImageR - Image model where red result is stored.
        destImageG - Image model where green result is stored.
        destImageB - Image model where blue result is stored.
        srcImg - Source image model.
    • Method Detail

      • finalize

        public void finalize()
        Prepares this class for destruction.
        Overrides:
        finalize in class AlgorithmBase
      • getFWHM

        public int getFWHM()
        returns the full width at half maximum of the autocovariance.
        Returns:
        fwhm
      • getFWHMB

        public int getFWHMB()
        returns the full width at half maximum of the blue autocovariance.
        Returns:
        fwhmB
      • getFWHMG

        public int getFWHMG()
        returns the full width at half maximum of the green autocovariance.
        Returns:
        fwhmG
      • getFWHMR

        public int getFWHMR()
        returns the full width at half maximum of the red autocovariance.
        Returns:
        fwhmR
      • calcStoreInDest2D

        private void calcStoreInDest2D()
        This function calculates the autocovariance coefficients and places them in the destination image for black and white images.
      • calcStoreInDest2DC

        private void calcStoreInDest2DC()
        This function calculates the autocovariance coefficients and places them in the destination images for color images.
      • calcStoreInDest3D

        private void calcStoreInDest3D()
        This function calculates the autocovariance coefficients and places them in the destination image for black and white images.
      • calcStoreInDest3DC

        private void calcStoreInDest3DC()
        This function calculates the autocovariance coefficients and places them in the destination images for color images.
      • calcStoreInDest4D

        private void calcStoreInDest4D()
        This function calculates the autocovariance coefficients and places them in the destination image for black and white images.
      • calcStoreInDest4DC

        private void calcStoreInDest4DC()
        This function calculates the autocovariance coefficients and places them in the destination images for color images.